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Wash Free Calcium Assay Kit
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HDB Wash Free Calcium Assay Kit
No-wash homogeneous calcium influx assay; Just mix and read.
HDB introduces a WASH Free Calcium Assay kit developed specifically to meet the requirements of high-throughput screening (HTS) applications. With this kit, calcium assays become a mix-and-read procedure in which cells are incubated with the kit reagents for one hour and transferred directly to the plate reader for evaluation. There are no intermediate wash steps involved. Eliminating the wash steps results in a lower variability and higher Z factors. It also makes the assay easier and faster, thus providing a fast, simple and reliable assay format for detecting changes in intracellular calcium with a low cost.
Applications
The kit provides a homogeneous assay for calcium flux. It is specially designed to work for a broad range of targets, including GPCRs and ion channels. The kits have been successfully used with both adherent and suspension cells.
Key features and benefits
No need to remove culture medium
No wash steps involved
Higher sensitivity
Cost effective
Ideal for all calcium influx assays
Adaptable to high throughput screening
Calcium influx assay principle
This kit provides a homogenous assay format for detection of calcium mobilization. Cells expressing a GPCR of interest or of channels that signal through calcium are pre-loaded with a calcium-sensitive dye and are then treated with compound. Upon stimulation, the receptor signals the release of intracellular calcium, which results in a large fluorescence increase of the calcium sensitive dye upon binding to calcium. The signal generated in the assay is read using fluorescent plate readers that possess an injector for the addition of agonist and is capable of a rapid read of the resulting change in fluorescence. The assay has been designed to accommodate both 96 well and 384 well formats, which are commonly used in high throughput cell-based functional screening.
Kit contents
HDB Calcium Assay Solution A
HDB Calcium Assay Solution B
Fluo-3/AM
Probenecid
Instrument requirement
Fluorescence plate reader with on board injectors
Learn more and purchase
Kits are available in two sizes providing sufficient material for either 10 plates (96, 384, or 1536 well) or 100 plates. Additionally, for higher volume customers, custom packaged materials can also be provided upon request. Please contact to: operation@hdbiosciences.com or call us at (+86)-21-58558330
 Description  Catalog No.  Size
 HDB WASH Free Calcium Assay Kit  HD02-0010  10 Plates
 HDB WASH Free Calcium Assay Kit  HD01-0100  100 Plates
Better signal, better performance
Fig 1. HEK293 cells response to ATP (10mM)
Fig 2. Comparison of HDB WASH Free Calcium Assay Kit and a competitive product.
Calcium influx induced by 20nM SubstanceP on HEK/SPR cell line. HDB WASH Free Calcium Assay kit provides a better signal over background than a leading competitive kit.
Fig 3. HDB WASH Free Calcium Assay Kit shows consistent pharmacology for known agonist and antagonist.
HEK/H3R/Ga15 Dose Response Curves. Cells were stimulated with Histamine agonist (left) or Clobenpropit antagonist (right)
over the indicated concentration ranges.
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